Our expertise in cell testing
Our efficiency studies carried out on cellular models provide evidence-based data. They are quick, less expensive, and more ethical than animal-based studies.
Antioxidant efficacy assays
We propose various cellular tests to measure the antioxidant activity and the effective concentrations by targeting the main mechanisms of action.
Targeted claims- Antioxidant effect
- Cellular free radical quenching
Measurement of the scavenging activity of intracellular ROS (Reactive Oxygen Species)
AOP 1
Targeted claims- Antioxidant effect
- Anti-aging effect
- Repairing
- Detox
- Cytoprotection
Measurement of the induction activity of the ARE (Antioxidant Response Element) pathway regulated by Keap1/Nrf2.
AOP 2
Targeted claims- Antioxidant effect
- Cellular lipid peroxidation quenching
Measurement of ROS scavenging activity at the plasma membrane
AOP 3
Targeted claims- Antioxidant effect
- Cellular ROS scavenging
- Catalase-like effect
Measurement of catalase-like activity (H2O2 scavenging activity).
AOP CAT
The 4 cellular assays AOP1, AOP2, AOP3 and AOP CAT ensure a complete study of the mechanism of action involved in the antioxidant effect.
The total cellular antioxidant efficacy score is deduced from the combination of the 4 assays.
287
Quercetin 1 mM score after profiling by mechanism of action (4 tests) on human keratinocytes
x7.91
Level of increase in expression of genes associated with the ARE/Nrf2 pathway after sulforaphane 12.5 µM 17h treatment on human keratinocytes
93.9%
Percentage neutralisation of free radicals produced by human HepG2 cells treated with 125 µM resveratrol
Other live cell assays
AOP POL
Measurement of the anti-pollution effect by scavenging ROS generated by urban dust
AOP TRANS
In vitro assessment of intestinal absorption by trans-epithelial transport
AOP UVA
Measuring protection against UVA radiation
We carry out in vitro cell tests for cosmetics (skin and hair), nutrition (human and animal), food supplements and health.
